human kidney cancer cell line achn  (Procell Inc)

Journal: BMC Systems Biology

Article Title: The anticancer mechanism investigation of Tanshinone II A by pharmacological clustering in protein network

doi: 10.1186/s12918-018-0606-6

Figure Lengend Snippet: The inhibitory effects of Tan IIA on proliferation of various cell lines. ( a - e ) Dose response curve of Tan II A treated Hela, MD-MB-231, HepG2, A549, ACHN cell lines. The cells were contineously incubated with test compound for 48 h. DMSO treated sample was determined as negative control. Doxorubicin (DOX, 10 μM) sample was defined as positive control. Data represented mean ± SEM, n = 4

Article Snippet: Cell culture Human non-small-cell lung cancer cell line A549, human kidney cancer cell line ACHN, human hepatocellular carcinoma HepG2 and human triple-negative breast cancer cell line MDA-MB-231 were derived from ATCC and cultured in Dulbecco’s modified Eagle medium (DMEM; HyClone Laboratories, Logan, UT, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Shanghai, China) and 1% Penicillin-Streptomycin solution.

Techniques: Incubation, Negative Control, Positive Control

Journal: Oncotarget

Article Title: Repression of PLA2R1 by c-MYC and HIF-2alpha promotes cancer growth

doi:

Figure Lengend Snippet: (A) PLA2R1 transcript levels were assayed by qRT-PCR and normalized against ACTB in five RCC cell lines: ACHN, Caki-2, RCC4, RCC10, and 786-O. VHL function is indicated for each cell line. (B) PLA2R1 transcript levels were assayed by qRT-PCR and normalized against ACTB in 786-O and (C) RCC10 control or constitutively expressing VHL. Histograms show an experiment representative of three independent experiments. (D-F) Cells were infected with a control retroviral vector or one encoding a VHL-resistant HIF1α mutant or a VHL-resistant HIF2α mutant. Infected cells were puromycin-selected. RNAs were prepared and reverse transcribed. Quantitative PCR was performed against PLA2R1 and normalized with respect to the ACTB mRNA level in (D) ACHN cells, (E) in RCC10+VHL cells and (F) in 786-O+VHL cells as indicated.

Article Snippet: The human kidney cancer cell lines ACHN, Caki-2, RCC4, RCC10, and 786-O were obtained from ATCC and virus-producing GP293 cells from Clontech.

Techniques: Quantitative RT-PCR, Control, Expressing, Infection, Retroviral, Plasmid Preparation, Mutagenesis, Reverse Transcription, Real-time Polymerase Chain Reaction